Antimicrobial and Antiproliferative Activity of Green Synthesized Silver Nanoparticles Using Bee Bread Extracts

Bee bread (BB) is a fermented mixture of bee pollen, rich in proteins, amino acids, fatty polyphenols, flavonoids, as well other bioactive compounds, and considered functional food for humans. In this study, we explored an innovative green synthesis colloidal silver nanoparticles, using BB extracts reducing stabilizing agents. A preliminary chemical characterization the was conducted. The plasmonic response as-synthesized nanoparticles (BB-AgNPs) evaluated by UV–Vis spectroscopy, while their hydrodynamic diameter zeta potential were investigated dynamic light spectroscopy (DLS). Transmission electron microscopy (TEM) analysis pointed out polydisperse NPs with quasi-spherical shapes. newly synthesized showed good antioxidant activity against tested free radicals, DPPH, ABTS•+, FRAP, best results being obtained case ABTS•+. BB-AgNPs exhibited antibacterial on Gram-positive Gram-negative bacterial strains: herein S. aureus, B. cereus, E. faecalis, coli, P. aeruginosa, enteritidis, yeast C. albicans, respectively. inhibition diameters varied between 7.67 ± 0.59 22.21 1.06 mm, values minimum inhibitory concentration 0.39 6.25 µg/mL. vitro antiproliferative colon adenocarcinoma, ATCC HTB-37 cell line, have shown that synthetized induced substantial decrease tumor viability dose-dependent manner IC50 ranging from 24.58 to 67.91 Consequently, more investigation required comprehend processes cytotoxicity AgNPs develop strategies mitigate potentially harmful effects harnessing antimicrobial properties.